RESUMEN
Background: In our previous study, the extract of Trachyspermum copticum [L.] Link seeds on gene expression of IFN-alpha and TGF-beta1 in mouse model with irritant contact dermatitis [ICD], in comparison with cutaneous corticosteroids were evaluated. In that study, in addition to significantly increase of IFN-alpha and TGF-beta1 genes expression levels in skin samples of [mice with ICD] groups treated with extract in comparison to other groups, histopathologic findings showed substantial improvement of skin color, texture and thickness, and also significant increase in hair follicle number. Therefore, we have decided to study the levels of Interleukin-1 [IL-1] gene expression, which plays a major role in inflammation responses, and Keratinocyte Growth Factor/Fibroblast Growth Factor-7 [KGF/FGF-7], which has growth effect on cells and is an important endogenous mediator of hair follicle growth and development
Materials and Methods: We used autopsy samples of skin lesions obtained from [mice model with irritant contact dermatitis [ICD]] from the previous study. In that study, [mice with ICD] divided in 9 groups and were treated with three concentrations of Trachyspermum Copticum [L.] Link dried seeds, cutaneous hydrocortisone, and fluocinolone acetonide. Then from the first day until the 10th day of treatment, clinical signs and histopathologic investigations were investigated. In the present study, using Real-Time PCR, the levels of IL-1 and KGF/FGF-7 genes expression in skin samples of inflammation site in above mice groups were studied. Statistical analysis, using one -way ANOVA, were performed. Level of significance was set at 0.05
Results: The IL-1 gene expression showed a significant difference between groups: IL-1 gene expression levels in mice with ICD treated with extract and corticosteroids were higher than the other groups [p=0.0001]. While in untreated [mice with ICD], no significant differences were observed. Also, during the treatment, there was a considerable increase in levels of IL-1 gene expression in groups treated with the extract at a rate of at least 2 to 3-fold in comparison with the [healthy untreated mice] group. The levels of KGF/FGF-7 gene expression in [mice with ICD] groups treated with the extract showed significance difference [p=0.014]; also there was a meaningful difference in [mice with ICD] groups treated with cutaneous corticosteroids [p=0.004]. While, in [untreated mice with ICD] group there were a significant decrease in the levels of KGF/FGF-7 gene expression in comparison with [healthy untreated mice] group [p=0.0001]. Also, changes in the levels IL-1 and KGF/FGF-7 gene expressions in each group in different days were seen
Conclusion: In this study, significant changes in the IL-1 and KGF/FGF-7 genes expression levels in the skin samples with inflammation, were associated with an increase in the rate and speed of improvement of contact dermatitis, more favorable conditions of the healed skin [in terms of color, consistency, and thickness], and a remarkable increase in the number of hair grown on the site of dermatitis [compared with control groups, and even groups with corticosteroid therapy]
RESUMEN
Background: The effectiveness of T cell vaccination has been demonstrated in a variety of animal models of both induced and spontaneous autoimmune diseases. The purpose of this study was to test the T cell vaccination protocol to treat and prevent collagen induced arthritis [CIA] in a rheumatoid arthritis model
Methods: CIA was induced by an intradermal injection of an artheritogen substance at the right paw of each female Albino rat under ether anesthesia. T cells were achieved from spleens of syngeneic rats that developed full clinical features of CIA. Rats suffering from CIA were divided in case groups [4 rats/group] based on the degrees of their disease and were injected intraperitoneally once with a suspension of T cells to investigate the effects of autoreactive T cells on CIA. To investigate the preventive effects of autoreactive T cells on CIA, 12 normal rats were injected intraperitoneally once either with a suspension of T cells or PBS, respectively. The results were evaluated by clinical observation, histopathological and radiographic findings
Results: Intraperitoneal inoculation of T cells to rats suffering from CIA, suppressed the development of CIA in case rats in stage 2 of the disease but not the other case rats. Rats who received T cells as prevention, showed the mild signs of disease. Injection of artheritogen substance to the case rats didn't result in development of CIA but the control rats, showed signs of CIA
Conclusion: The results of this pilot study demonstrate that CIA presentations and signs can be subsided or suppressed by autoreactive T cells. The vaccination is most effective before onset of the disease and in early phases of CIA. Modifying and improving the protocol using more cases is recommended
RESUMEN
Type 1 Diabetes [T1D] is a chronic and progressive autoimmune disorder. Cytokines play a critical role in the pathogenesis of T1D. IFN- polymorphism was investigated in T1D and compared with normal controls. Thirty patients suffering fromT1D and 40 normal controls were studied simultaneously using PCR technique. IFN- gene was evaluated at position 5'UTR +5644. There was a significant difference between patient and control groups in TT genotype [P<0.05]. In this study, we found a negative association between IFN- gene at position 5'UTR +5644 and T1D in Iranian patients pointing to T allele as a protective factor against T1D